OGG1 is essential in oxidative stress induced DNA demethylation

“…This increase in AID can explain how DNA demethylation occurs once the TET enzymes oxidize 5mC to 5hmC. This result was similar for the OGG1 expression, which has been recently revealed as the last major effector in the process of demethylation ( 44 ).…”

“…Cells induce the transcription of an array of genes in response to ROS and ROS‐induced DNA damage. We monitored across the O 2 spectrum the expression of 7 genes encoding DNA damage response and antioxidant proteins: OGG1 (18), PARP‐1 (19), X RCC4 (20), Cu/Zn‐SOD (21), NQO1 (22), SRXN1 (23), and TXNRD1 (24). In U87MG, genes encoding DNA repair enzymes OGG1 and PARP‐1 displayed 5–15‐fold significant reduction in expression at 5% O 2 relative to normoxia, and 3‐fold significant increase in expression in the reoxygenation control relative to normoxia ( Fig.…”

“…Reactive oxygen species (ROS) such as superoxide anion, hydrogen peroxide, and hydroxyl radical are involved in various important bio-functions such as cell signaling, metabolism, homeostasis, and formation of memory through the methylation of DNA [ 66 , 67 ]. However, the overexpression of ROS can lead to the development of neurodegenerative diseases [ 68 ].…”

“…This increase in AID can explain how DNA demethylation occurs once the TET enzymes oxidize 5mC to 5hmC. This result was similar for the OGG1 expression, which has been recently revealed as the last major effector in the process of demethylation ( 44 ).…”

“…Cells induce the transcription of an array of genes in response to ROS and ROS‐induced DNA damage. We monitored across the O 2 spectrum the expression of 7 genes encoding DNA damage response and antioxidant proteins: OGG1 (18), PARP‐1 (19), X RCC4 (20), Cu/Zn‐SOD (21), NQO1 (22), SRXN1 (23), and TXNRD1 (24). In U87MG, genes encoding DNA repair enzymes OGG1 and PARP‐1 displayed 5–15‐fold significant reduction in expression at 5% O 2 relative to normoxia, and 3‐fold significant increase in expression in the reoxygenation control relative to normoxia ( Fig.…”

“…Reactive oxygen species (ROS) such as superoxide anion, hydrogen peroxide, and hydroxyl radical are involved in various important bio-functions such as cell signaling, metabolism, homeostasis, and formation of memory through the methylation of DNA [ 66 , 67 ]. However, the overexpression of ROS can lead to the development of neurodegenerative diseases [ 68 ].…”

“…This increase in AID can explain how DNA demethylation occurs once the TET enzymes oxidize 5mC to 5hmC. This result was similar for the OGG1 expression, which has been recently revealed as the last major effector in the process of demethylation ( 44 ).…”

“…Cells induce the transcription of an array of genes in response to ROS and ROS‐induced DNA damage. We monitored across the O 2 spectrum the expression of 7 genes encoding DNA damage response and antioxidant proteins: OGG1 (18), PARP‐1 (19), X RCC4 (20), Cu/Zn‐SOD (21), NQO1 (22), SRXN1 (23), and TXNRD1 (24). In U87MG, genes encoding DNA repair enzymes OGG1 and PARP‐1 displayed 5–15‐fold significant reduction in expression at 5% O 2 relative to normoxia, and 3‐fold significant increase in expression in the reoxygenation control relative to normoxia ( Fig.…”

“…Reactive oxygen species (ROS) such as superoxide anion, hydrogen peroxide, and hydroxyl radical are involved in various important bio-functions such as cell signaling, metabolism, homeostasis, and formation of memory through the methylation of DNA [ 66 , 67 ]. However, the overexpression of ROS can lead to the development of neurodegenerative diseases [ 68 ].…”